Coding

Part:BBa_K2603002:Design

Designed by: Cibele Zolnier Sousa do Nascimento, Fernando Satoshi Tutihashi   Group: iGEM18_USP-EEL-Brazil   (2018-10-09)


Lac PL - Laccase from Pleurotus ostreatus with LacI promotor and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1011
    Illegal BamHI site found at 694
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1202


Design Notes

The restriction sites for SpeI, PstI, XbaI and EcoRI from the gene sequence.


Source

Gene sequence from Pleurotus ostreatus NRRL0366 taken from NCBI, RBS ans promotor sequences from iGEM's Registry.

References